An interactive web-based box plot and significance annotation tool supporting automatic wide-to-long data reshaping, multiple statistical tests (t-test/Wilcoxon), customizable significance labels, and publication-quality black-white themes (theme_bw).
Automatically transform wide-format data to long-format (melt) without manual preprocessing. Upload your data and start analyzing immediately.
Support t-test (parametric) and Wilcoxon rank-sum (non-parametric) tests. Automatic p-value calculation with significance bars and step_increase overlap prevention.
Classic black-and-white theme (theme_bw) with optional 90-degree X-axis label rotation to prevent overlapping, meeting journal publication requirements.
Configure box plot and significance testing parameters
Wide format (one column per sample) or long format
Select two groups for comparison. P-values are calculated automatically with connecting lines.
Statistical test: t-test
Upload a 3-column TSV file (id, Type, expression) and click Generate to create boxplot